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What is
it ? A
sclerotium (plural: sclerotia) is a hardened mass of mycelium which is more
resistant to adverse environmental conditions than normal mycelium. Sclerotia
form from the mycelium of certain mushroom species as a defense mechanism
against dryness, cold, heat, or excessive moisture. Sclerotia contain
approximately 30% dry matter. The potency of dry sclerotia is around 2/3 that
of dry Psilocybe cubensis fruitbodies. (As with
every mushroom, the potency can vary depending on the mushroom strain,
substrate composition and other factors) What
species form sclerotia? Psilocybe
mexicana and Psilocybe tampanensis both form sclerotia. The “A” strain of P. Mexicana
in particular is known for its exceptional sclerotia forming abilities. How are
sclerotia grown? Sclerotia
can be grown in vitro on rye grass seed or different grains, or harvested from
spent casings of P. mexicana or P. tampanensis. Growing
sclerotia in vitro is very simple and does not require any of the terrariums,
air exchanges, or other complexities of fruiting mushrooms, but takes longer.
Different grain substrates will work for sclerotia, but PF substrate is not
suitable because of its vermiculite content. Any substrate containing materials
not fit for human consumption should not be used for growing sclerotia, as it
may be difficult during harvesting to separate the sclerotia from the
substrate. Rye grass
seed (without fungicides) produces the highest yields of sclerotia, and so is
the preferred substrate. In addition, some vendors sell ready made
pre-sterilized bags of substrate in which sclerotia can be grown. Preparing
jars for: Substrate
for sclerotia formation for Psilocybe mexicana , Psilocybe tampanensis and
Psilocybe atlantis Substrate
for fruits of Psilocybe mexicana, Psilocybe tampanensis,Psilocybe semperviva,
Psilocybe atlantis ... Recipe #1
Rye Grass Seed Recipe QUART Jar
300 ml
Rye Grass Seed 150 ml
Water PINT Jar 150 ml
Rye Grass Seed 75 ml
Water Recipe #2
Whole white rice recipe QUART
jar: 300 ml
whole white rice 400 ml
water PINT jar: 150 ml
whole white rice 200 ml
water 1. Fill
jars with grain and water 2. Remove
and punch an inoculation hole in the lid of each jar. 3. Screw
a lid fitted with a Tyvek or polyfill filter on each jar, shake well to
distribute the seeds and water. In
addition an inoculation hole can be made, where you can inoculate the jars with
a syringe. Cover the hole with a piece of tape. 4.
Sterilize in pressure cooker for 1 hour at 15 PSI. 5. Shake
well while the jars are still hot to distribute the wettter and dryer seeds
evenly. Place back into the pressure cooker to cool down. 6. When
jars have cooled completely, they may be inoculated. Inoculate with a spore
syringe containing P. mexicana or P. tampanensis spores (preferably P. mexicana
strain “A”, and make sure to flame-sterilize the needle) or liquid inocúlate
prepared from a actively growing culture on agar. Incubation The jars
are then left in a dark place between 70 and 80 °F. They should be shaken 1-2
times during the first few weeks to spread the growing mycelium evenly. After
they are fully colonized, sclerotia will begin to form in the jars. They should
not be shaken after this point. Sclerotia will continue to form for 2 to 3
months after complete colonization. Time from inoculation to harvest is
approximately 3 or 4 months. Harvest After
sufficient time has passed and ample sclerotia have formed, it is time to
harvest. This is done by picking through the jars and obtaining all the
sclerotia. When first harvested, there will be bits of grain and mycelia
sticking to the sclerotia. They can be cleaned with a soft toothbrush and
water. Fresh sclerotia should be dried in the same manner as fresh mushrooms.
It may be necessary to cut some of it into smaller pieces for this. Sclerotia
consist of about 30% dry matter. Yields of more than 30 grams of dry sclerotia
per cup of substrate have been reported when growing P. mexicana on rye grass
seed. At 2/3 the potency of dry cubensis, this is equivalent to 20 grams of
regular dry cubensis per cup of substrate. How can I
eat sclerotia? Like
mushrooms, sclerotia can be eaten dry, fresh, or re-hydrated, but should not be
cooked. It has a consistency like peanuts. Good results can be obtained from
substituting sclerotia for nuts in any no-cook recipe. How does
the potency of P. mexicana and P. tampanensis sclerotia compare to Psilocybe
cubensis fruitbodies? Sclerotia
is roughly 70% water by weight while mushrooms are around 90% water. Dry
sclerotia is 2/3 as potent as dry cubensis fruits, while fresh sclerotia is
twice as potent as fresh cubensis. This is due to the fact that sclerotia
contain 3 times more dry matter per quantity of fresh material. |